Study on Mechanism of Sophora flavescens against hepatitis B virus / 中国生化药物杂志

ABSTRACT

Objective To study on Sophora flavescens against hepatitis B virus (HBV) and its mechanism.Methods HepG2.2.15 cells were cultured in vitro, respectively with the negative control group without drug intervention, positive control group (5-Fu group) which was given 0.77 mmol/L 5-Fu, Matrine group treated with 0.77 mmol/L Matrine intervention.HBSAg, HBeAg inhibition rate and cell survival rate, apoptosis rate, cell cycle as observation indexes, comprehensive evaluation of matrine effect on anti 2.2.15 cell HBV in vitro.Results The positive control group, matrine group compared with the negative control group, the inhibition rate of HBsAg and HBeAg were statistically difference ( all P<0.05 ) , when positive control group and Oxymatrine group compared, the inhibition of HBsAg and HBeAg, the difference were statistically (all P<0.05).Cell survival rate, when the positive control group and the negative control group compared, had no significant difference (P<0.05), when matrine group compared with the negative control group, there were significant differences in the survival rate of the cells (t=4.25, P<0.05).Rate of apoptosis cells in negative control group and positive group had not statistically significant, matrine group apoptosis rate was higher than that of the negative control group and the control group (P<0.05).The cells in G0/G1 phase, had no significant differences of distribution in each group, the cells in G2/M phase, the positive control group and matrine group were lower than those in negative control group (P<0.05), matrine group was lower than that of the positive control group (P<0.05).The S phase cells of positive control group and matrine group were higher than those in negative control group (P<0.05), matrine group was higer than that of the positive control group (P<0.05).Conclusion Matrine has obvious effect of anti HBV, inducing cell apoptosis, cell proliferation arrest in S phase.