Expression of estrogen receptor and progesterone receptor and their significance in the endo-metrial tissue of intrauterine adhesion patients / 临床与实验病理学杂志

ABSTRACT

Purpose To investigate the expression of estrogen receptor ( ER) and progesterone receptor ( PR) in endometrial tissue in patients with intrauterine adhesion ( IUA) and their clinical significance. Methods Two experimental methods, immunohistochemical MaxVision two step method and real-time fluorescencequantitative PCR ( qRT-PCR) , were used for detection of ER and PR in endome-trial tissue both in group IUA ( study group) and non IUA group ( control group) . Results MaxVision immunohistochemical method showed that ER protein expression in the study group (3. 52 ± 0. 71) was significantly higher than that (2. 75 ± 1. 00) in the control group (P=0. 01), ER mRNA expression by qRT-PCR was also significantly higher in the study group (1. 59 ± 0. 26) than that (1. 00 ± 0. 19) in the control group (P=0. 00). The immunohistochemical detection of showed that PR protein expression in the study group (3.26 ±0.70) had no significant different from that (3.58 ±0.28) in the control group (P=0.12), qRT-PCR also showed that ER mRNA in the study group (1.15 ±0.21) had no significantly different from that (1.00 ±0.31) in the control group (P=0. 21 ) . Immunohistochemical expression of ER and PR proteins showed a significant difference between the endometrial glands and en-dometrial stroma (χ2 =5. 797, P=0. 016,χ2 =4. 857, P=0. 027). Conclusion The expression of ER in endometrial tissue in the study group is higher than that in the control group, but the expression of PR has no different between the two groups, ER and PR pro-teins expression are higher in endometrial glands than that in endometrial stroma. These provide a theoretical reference for clinical use of estrogen and progesterone to IUA patients.