Effects of Tianqi Yizhi Granules on Mitochondria of Brain Tissues and Energy Metabolism ;in Alzheimer Rats / 中国中医药信息杂志

ABSTRACT

Objective To investigate the action mechanism of Tianqi Yizhi Granules for Alzheimer disease. Methods Ninety SD rats were divided into sham-operation group, model group, positive medicine group, high, medium and low dosage of Tianqi Yizhi Granules groups. SD rats were injected with aggregated amyloid β1-42 protein (Aβ1-42) into their left ventricle to establish AD models. Treatment groups received gavage with Huperzine A or Tianqi Yizhi Granules. The sham-operation group and model group received gavage with the same volume of normal saline. The mitochondrial membrane potential, activity of complex Ⅰ, Ⅱ, Ⅲ and Ⅳ of mitochondrial respiratory chain, enzymatic activity of Na+-K+ATP, energy charge, and space leaning ability were detected. Results Compared with model group, Tianqi Yizhi Granules could significantly improve mitochondrial membrane potential, activity of complex Ⅰ, Ⅲ and Ⅳ of mitochondrial respiratory chain, enzymatic activity of Na+-K+ATP, space leaning ability, and energy metabolism of brain in AD rats. Conclusion Tianqi Yizhi Granules showed mitochondrial protective capacity, and could improve energy metabolism of brain in AD rats, and then improve AD rats’ space learning ability.