Effects of Ginkgo Biloba Extract on Proliferation of Non-small Cell Lung Cancer PC-9 Cells / 中国中医药信息杂志

ABSTRACT

Objective To investigate the proliferation inhibitory effect and mechanism of Ginkgo Biloba extract (EGB) on non-small cell lung cancer (NSCLC) PC-9 cells. Methods Concentrations of EGB were set as 70 mg/L, 105 mg/L, 140 mg/L, 210 mg/L, and 280 mg/L, and were used to culture PC-9 cells in vitro for 24 h, 48 h, and 72 h. Tetramethylazo thiazole blue staining (MTT) method was applied to detect cell inhibition rate. Hoechst33258 fluorescent staining was employed to observe the nucleus. SOD activity and MDA content were detected by ELISA. The protein expressions of Caspase-3, Caspase-9, Cyt-C, and AIF were detected by Western blot. Results After incubated for 24 h, 48 h, and 72 h, EGB inhibited the proliferation of PC-9 cells, IC50 was 195.45 mg/L, 179.63 mg/L, 142.23 mg/L, respectively). 105 mg/L, 140 mg/L, 210 mg/L EGB could induce apoptosis of PC-9 cells, cause the nucleus pycnosis, produce apoptotic bodies, improve SOD activity and decrease MDA content (P<0.05, P<0.01, P<0.001). Western blot results showed that, compared with the control group, EGB can obviously increased the protein expressions of Caspase-3, Caspase-9, Cyt-C, and AIF, with statistical significance (P<0.05, P<0.01, P<0.001). Conclusion EGB can effectively restrain proliferation of non-small cell lung cancer PC-9 cells, the mechanism may be realized by inducing PC-9 cell apoptosis through pathway of mitochondria apoptosis, reducing oxidation, and clearing free radicals.