Stem cell culture technology：innovation, breakthrough and high quality / 中国组织工程研究

ABSTRACT

BACKGROUND:With the development of fundamental research and clinical research on stem cels, the quantity demanded for stem cels is on the increase, but the relatively out-dated cultivating technology of stem cels restrict the development of the research on stem cels. OBJECTIVE: To review the improvement and innovation of stem cel culture techniques both at home and abroad. METHDOS In the title and abstract, “stem cels, induced pluripotent stem cels, cultivation, efficiency, reprogramming” in Chinese and English chosen as search terms were utilized to search for the papers related to stem cel culture techniques published from January 2005 to November 2014 in CNKI, VIP, Wanfang and PubMed databases, respectively. In the same field, articles published lately in the authoritative journals were preferred. Totaly 61 articles were searched initialy, and only 39 articles were included in result analysis. RESULTS AND CONCLUSION: The development and improvement of stem cel culture techniques effectively increase the culturing quantity of stem cels, improve the development of basic and clinical studies on stem cels. With the further innovation and breakthrough of stem cel culture techniques, it wil be possible to efficiently culture high-quality stem cels, and the stem cel transplantation tests for human diseases wil be promoted greatly.