Silence Yes-associated protein expression inhibit the invasion ability of hepatocellular carcinoma cells / 中华肝胆外科杂志
Chinese Journal of Hepatobiliary Surgery
; (12): 805-809, 2014.
Article
in Zh
| WPRIM
| ID: wpr-466296
Responsible library:
WPRO
ABSTRACT
Objective To investigate the correlation between the Yes-associated protein (YAP) expression and epithelial-mesenchymal transition (EMT) by transiently transfecting with YAP small-interfering RNA (siRNA) in MHCC97H and MHCC97L cells.Methods MHCC97H and MHCC97L cells were transiently transfected by YAP siRNA.Furthermore,protein expressions and mRNA levels of characteristic markers of EMT (E-cadherin,N-cadherin) were examined by Western blotting and real-time polymerase chain reaction.Transwell invasion assay was used to detect changes of invasiveness of MHCC97H and MHCC97L cells.Results The YAP siRNA transfected group in MHCC97H was examined after 72 hours by Western blotting.The result showed obviously higher expression of E-cadherin in transfected group compared with the control group (P < 0.05),and lower expression of N-cadherin (P < 0.05).In MHCC97L cells,the expression of E-cadherin was also significantly increased (P < 0.05),however,N-cadherin expression did not significantly change (P > 0.05).Moreover,compared with the control group,transwell invasion assay showed that the number of the transfected group cells significantly decreased in MHCC97H(66 ± 6.89 vs 117 ± 7.23,P < 0.05),and compared with the control group,the number of the transfected group also significantly decreased in MHCC97L (40 ±2.65 vs 77 ±4.33,P <0.05).The result of real-time polymerase chain reaction indicated that mRNA levels of E-cadherin increased (P < 0.05),but mRNA levels of N-cadherin did not significantly change (P > 0.05).This is considered as post-transcriptional regulation after silencing YAP in MHCC97H and MHCC97L.Conclusions YAP silencing is able to inhibit EMT in MHCC97H and MHCC97L cells by modulating the characteristic markers of EMT.The inhibition of YAP expression can reduce the invasion ability of hepatocellular carcinoma cells.
Full text:
1
Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Hepatobiliary Surgery
Year:
2014
Type:
Article