Mechanism of immunotolerence induced by bone mesenchymal stem cell co-transplanted with islets through regulating Tfh in NOD mice / 中华肝胆外科杂志

ABSTRACT

Objective To study the regulation mechanism of bone mesenchymal stem cell (MSC)combined co-translation of islets in differentiation of Follicular Helper T cell (Tfh),and its roll on immunotolerence induction in non-obese diabetic (NOD) mice transplantation model.Methods The NOD mice were divided into 4 groupsGroup A with islet transplantation alone;Group B with MSC co-transplantation with islets (MSC0.5 × 106);Group C with MSC co-transplantation with islets (MSC2 × 106);Group D with MSC co-transplantation with islets (MSC3 × 106).ELISA was used to test the expression level of diabetes autoantibody GAD65Ab and IAA.Tfh cell count was detected by FACS.Results The survival time of transplantation groups was much longer in MSCs co-transplantation group than islet-alone group;the level of GAD65Ab,IAA and Tfh cell count were much lower in MSCs co-transplantation group than islet-alone group.Conclusion MSC may protect the islet transplants by regulating the Tfh cell differentiation.