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Expression of a proliferation-inducing ligand in B-cell chronic lymphocytic leukemia and its clinical significance / 白血病·淋巴瘤
Journal of Leukemia & Lymphoma ; (12): 156-159, 2014.
Article in Chinese | WPRIM | ID: wpr-466921
ABSTRACT
Objective To quantitatively analyze the mRNA and protein expression level of a proliferation-inducing ligand (APRIL) and investigate its clinical significance in peripheral blood mononuclear cells and plasma from newly diagnosed patients with B-cell chronic lymphocytic leukemia (B-CLL).Methods The mRNA of the target gene in 32 B-CLL patients and 15 health controls was quantified with real-time fluorescence quantitative polymerase chain reaction (RFQ-PCR) and protein by enzyme-linked immunosorbent assay (ELISA).Results APRIL mRNA was assayed with RFQ-PCR,the intra-and inter-batch reproducibility showed the coefficient of variation (CV) were 1.69 %-6.98 % and 6.49 %-10.27 %,respectively.The expressions of APRIL mRNA and protein in patients with B-CLL were significantly higher than those in control (P < 0.05),and significant difference was noted among the comparable stages in the arms (P < 0.05).The expression of APRIL mRNA and protein in TDI (treatment-demand-indicator) arm was significantly higher than those in non-TDI arm (P < 0.05).Conclusions APRIL may be involved in the formation and development of B-CLL and be an influence factor for disease staging.Thus,APRIL may be a prognostic indicator as well as the therapy target for the disease.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Prognostic study Language: Chinese Journal: Journal of Leukemia & Lymphoma Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Prognostic study Language: Chinese Journal: Journal of Leukemia & Lymphoma Year: 2014 Type: Article