Analysis on role of dependence of stromal cell derived factor-1 on myocardial infarction angiogenesis and function recovery / 重庆医学

ABSTRACT

Objective To study the role of dependence of stromal cell derived factor‐1(SDF‐1) on myocardial infarction an‐giogenesis and function recovery .Methods The rats were randomly divided into the test group (65 cases) and the treatment group (64 cases) ,in which 5 rats in the test group were randomly killed on 1 ,2 ,4 ,7 ,14 ,28 d before and after myocardial infarction(infarc‐tion group) and the SDF‐1 protein level was detected .At the same time the sham operation group(5 cases) was set up;64 rats in the treatment group were randomly divided into four groups ,16 cases in each group ,which were infarction+SDF‐1 group ,infarct+anti‐SDF‐1 group ,infarct+normal saline group and normal+normal saline group .After the corresponding processing in each group ,the SDF‐1 levels after myocardial infarction ,MSCs homing amount ,neovascularization density ,EF and FS levels were compared among various groups .Results The SDF‐1 level after 1-7 d in the infarction group was significantly higher than that in the sham opera‐tion group ,and reached a peak level ,then decreased gradually and restored to normal on 14 d ,the difference was statistically signifi‐cant (P<0 .05) .The MSCs homing amount and neovascularization density level in the SDF‐1 treatment groups were significantly higher than those in other groups ,which in the infarction+anti‐SDF‐1 group were significantly lower than those in the infarction+normal saline group ,the differences were statistically significant (all P<0 .05) .The cardiac function improvement situation in the infarction +SDF‐1 group was significantly better than that in other groups ,the difference was statistically significant (P<0 .05) . Conclusion SDF‐1 can promote angiogenesis in myocardial infarction ,also improve the heart function .