Your browser doesn't support javascript.
loading
CD8α+α+ T cells in lesions and peripheral blood of patients with psoriasis / 中华皮肤科杂志
Chinese Journal of Dermatology ; (12): 229-232, 2015.
Article in Chinese | WPRIM | ID: wpr-468687
ABSTRACT
Objective To investigate the distribution and proportion of CD8α+α + T cells in lesions and peripheral blood of patients with psoriasis,and to assess their roles in the pathogenesis of psoriasis.Methods An immunofluorescence assay was performed to observe the distribution of CD8α+α+ T cells in lesions of 5 patients with progressive psoriasis vulgaris and normal skin of 5 healthy human controls.Flow cytometry was conducted to determine the proportion of CD8α+α+ T cells,and to measure the expressions of interferon (IFN)-γ and tumor necrosis factor (TNF)-α in peripheral blood from 10 patients with progressive psoriasis vulgaris and 8 healthy human controls.Statistical analysis was carried out by t test with GraphPad Prism software.Results A massive infiltrate mainly composed of CD8α+α+ T cells but not CD8α+β+ T cells was observed in the upper dermis of lesions from the 5 patients with psoriasis,while there was no infiltrate of CD8α+β+ or CD8α+α+ T cells in the normal skin of 5 healthy human controls.Flow cytometry revealed that the proportion of CD8α+α+ T cells was significantly higher in peripheral blood from 10 patients with psoriasis than in that from 8 healthy human controls (26.47% ± 12.99% vs.9.12% ± 4.80%,t =3.96,P< 0.001).Significant differences were also noted between the psoriatic patients and healthy human controls in the percentage of cells secreting IFN-γ (47.36% ± 19.38% vs.13.44% ± 9.21%,t =4.54,P < 0.001) and cells secreting TNF-α (54.14% ± 21.14% vs.34.03% ± 17.22%,t =2.17,P < 0.05) in peripheral blood CD8α+α+ T cells.Conclusions Both the distribution and proportion of CD8α+α+ T cells are increased in lesions and peripheral blood from patients with psoriasis,suggesting that CD8α+α+ T cells may be the main subgroup of CD8+ T cells that contribute to the pathogenesis of psoriasis.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Dermatology Year: 2015 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Dermatology Year: 2015 Type: Article