Research on dynamic changes of claudin-5 expression around hematoma of intracerebral hemorrhage in experimental rats / 中华老年医学杂志

ABSTRACT

Objective To investigate the dynamic changes of claudin-5 expression around hematoma of intracerebral hemorrhage (ICH) in experimental rats and its correlation with cerebral edema.Methods 108 adult male SD rats were randomly divided into 6 groupssham operation,6 h,12 h,24 h,3 d,7 d after operation groups respectively.The experimental ICH model was established by injecting autologous arterial blood into the caudate nucleus.Immunohistochemistry straining was used to detect the changes of expression of claudin-5 around hematoma at different time point after ICH.The permeability of blood-brain barrier (BBB) was tested by Evans blue method.The changes of brain water content were measured by the wet and dry weight method.Alexis method was used to examine the changes in neurological deficit scores.Results The expression of claudin-5 around hematoma was decreased gradually from 6 h after ICH,and it declined quickly at 12 h to 3 d after ICH.The expression of claudin 5 around hematoma was slightly lower at 7 d after ICH than in sham operation group.Permeability of the BBB was higher at 12 h to 3 d after ICH than any other groups,and reduced at 7 d after ICH.Brain water content was increased from 6h,the severest water content was at 12 h to 3 d after ICH,and it decreased gradually at 7 d after ICH.The neurologic impairment appeared from 6 h after ICH,the time point of severest neurologic impairment was at 12 h to 3 d after ICH,and it reduced from 7 d after ICH.The expression of claudin-5 around hematoma after ICH was negatively correlated with the permeability of BBB,brain water content and neurological deficit scores (r=-0.63,-0.71,0.59,all P＜0.05).Conclusions Claudin-5 expression plays an important role in the course of hemorrhagic cerebral edema and injury.It probably would he an effective way to alleviate the degree of hemorrhagic cerebral edema and injury after ICH by actively increasing the expression of claudin-5 at the early period of ICH.