Influence of Wnt signaling pathway on peroxisome proliferator-activated receptor γ and glucokinase expression in mice NIT-1 β-cell cultured in vitro / 中华内分泌代谢杂志
Chinese Journal of Endocrinology and Metabolism
;
(12): 990-994, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-469944
ABSTRACT
Objective To investigate the expression of peroxisome proliferator-activated rec eptor γ (PPARγ) and glucokinase (GK) induced by Wnt signaling pathway in mice NIT-1 β-cells,and to explore the interaction between PPARγ and Wnt signaling pathways.Methods Recombinant Wnt3a protein was applied to NIT-1 beta-cells to activate Wnt signaling pathway.The expression of PPARγ was determined by real-time PCR and Western blotting.The expression of GK was determined by real time PCR.Results Wnt3a rapidly activated Wnt/β-catenin/TCF signaling pathway,and increased PPARγ and GK mRNA expression by 41.2% and 65.0% in NIT-1cells,with PPARγ protein expression increasing by 97.8% (P<0.01).These effects were abrogated by Wnt and PIK3 inhibitors,dickkopf 1 and wortmannin treatment (P< 0.01).Conclusions PPARγ and GK can be upregulated by Wnt singnaling,and the effects might partially be PI3K-dependent.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Endocrinology and Metabolism
Year:
2014
Type:
Article
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