Comparison of Immune Responses Elicited by Recombinant Fusion Hapten and Coupled Hapten / 天津医药
Tianjin Medical Journal
;
(12): 862-864, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-471415
ABSTRACT
Objective:
To identify the immune characteristics elicited by immunogen prepared in two different ways against a 36AA-peptide. Methods:Rabbits were immunized by a 36-AA-hapten coupled with carrier proteins or recombinant fusion protein separately. The kinetics and specificities of antibody responses were compared. Results:After about 3 months of primary immunity, the antisera elicited by two kinds of immunogen reached the peak titer. The highest titer elicited by the immunogen prepared with coupled-hapten method reached 1∶12 000, while the highest titer elicited by the immunogen prepared with gene engineering method was 1∶6 000. But the immune response produced by the recombinant fusion protein was more long-lasting. Conclusion:Both kinds of immunogen can successfully elicit 36-AA specific antibody response in rabbits, which have different immune characteristics. To couple the hapten with vector proteins is a quicker and more effective way to prepare the immunogen.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Tianjin Medical Journal
Year:
2009
Type:
Article
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