Interleukin-6 Inhibits the Expression of Apolipoprotein M in HepG2 Cells / 天津医药
Tianjin Medical Journal
;
(12): 216-218, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-471584
ABSTRACT
ObiectiveTo investigate the effect of interleukin-6(IL-6)on apolipoprotein(apo)M expression in a human hepatoblastoma cell line(HepG2).Methods:
HepG2 cells were cultured and incubated with different concentrations of IL-6 (0,1.25,2.5,5,10,20,40 and 80 μg/L)for 24 hours.After the incubations,total RNAs were extracted and applied to reverse transcript PCR and real-time quantitative PCR to detect the expression levels of apoM and apoA-I.The effect of IL-1α on apoM expression was also determined.Results:
IL-6 significantly inhibited the expression of apoM(F=10.778,P < 0.01).Whereas IL-6 did not influence the expression of apoA-I(F=2.004,P > 0.05).IL-1α(0,1.25,2.5,5,10,20,40 and 80 μg/L)did not decrease the expression of apoM(F=2.038,P > 0.05).CooclusionIL-6 inhibits apoM mRNA transcription,which may contribute to the pathogenesis of abnormal lipid metabolism and macrovascular complications in type 2 diabetes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Tianjin Medical Journal
Year:
2010
Type:
Article
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