Proteomics Analysis of Component in Serum with Anti-asthma Activity Derived from Rats Treated by Acupuncture / 针灸推拿医学(英文版)
Journal of Acupuncture and Tuina Science
;
(6): 326-331, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-472489
ABSTRACT
Objective:
To identify the differential proteins in the serum with anti-asthma activity of acupuncture to pinpoint the substance basis of anti-asthma activity of acupuncture by proteomics method.Methods:
Two-dimensional electrophoresis of protein was employed to analyze the differential proteins in the serum with anti-asthma activity of acupuncture, and identify the differential proteins with mass spectrum technique.Results:
In adrenalectomy (ADX)-asthma acupuncture group and asthma acupuncture group, two-dimensional electrophoresis showed that there was an obvious fuscous point (MW=44.1kD, PI=5.1), which was 60S ribosomal protein L13, or hypothetical protein FLJ32191 analyzed by mass spectrum technique, different from other groups. In ADX-asthma acupuncture group, there were two obviously fuscous points (MW=39.SkD, PI=4.9 and MW=39.SkD, PI=5.0), which were zinc finger protein 208, and thyroid hormone receptor-associated protein analyzed by mass spectrum technique, and two special proteins (MW=17.3kD, PI<4.5 and 16.9kD, PI<4.5), which were Cyclophilin A and zinc finger protein 91 analyzed by mass spectrum technique, different from other groups.Conclusion:
There are multi-target effects regulating the whole body in acupuncture treating asthma relating to immunoloregulation, gene expression, and protein synthesization. The effects of acupuncture on the response proteins need further research.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Acupuncture and Tuina Science
Year:
2009
Type:
Article
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