Expression of Rous Sarcoma Virus Enhancer Factor Gene in Human Hepatocellular Carcinoma / 대한암학회지
Journal of the Korean Cancer Association
;
: 547-554, 1997.
Article
in Korean
| WPRIM
| ID: wpr-47359
ABSTRACT
PURPOSE:
We have previously cloned three enhancer factor genes encoding proteins that bind to long terminal repeats (LTRs) of Rous sarcoma virus. Among these genes, RSV- EF-I gene is expressed in rat hepatoma tissues and several proliferating cell lines but not in normal rat liver tissues. We have isolated the human homologue of RSV-EF-I gene and examined its expression in human hepatocellular carcinoma tissues. MATERIALS ANDMETHODS:
We have screened the human genomic library and cDNA library of Hep G2 cell line derived from human hepatocellular carcinoma to isolate the human homologue of RSV-EF-I gene.RESULTS:
We have isolated one cDNA clone containing about 1.5 kb insert and sequenced. Sequence analysis reveals that this human homologue of RSV-EF-I gene has a high similarities to human YB-1 mRNA, human DNA-binding protein B (dbpB) gene and other Y-box protein genes. It is expressed in human hepatocellular carcinoma but very slightly in normal human liver tissues in Northern blot analysis.CONCLUSION:
Our data suggest that the human homologue of RSV-EF-I gene presumably belongs to Y-box protein family genes and plays a role in the transformation of the human hepatoma cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Gene Library
/
Genomic Library
/
Cell Line
/
Blotting, Northern
/
Clone Cells
/
Sequence Analysis
/
DNA, Complementary
/
Sarcoma, Avian
/
Carcinoma, Hepatocellular
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Journal of the Korean Cancer Association
Year:
1997
Type:
Article
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