Simultaneous determination of borneol and its metabolite in rat plasma by GC-MS and its application to pharmacokinetic study / 药物分析学报
Journal of Pharmaceutical Analysis
; (6): 345-350, 2014.
Article
in Zh
| WPRIM
| ID: wpr-473727
Responsible library:
WPRO
ABSTRACT
A gas chromatography mass spectrometry (GC-MS) method has been developed and fully validated for the simultaneous determination of natural borneol (NB) and its metabolite, camphor, in rat plasma. Following a single liquid-liquid extraction, the analytes were separated using an HP-5MS capillary column (0.25 mm ? 30 m ? 0.25μm) and analyzed by MS in the selected ion monitoring mode. Selected ion monitor (m/z) of borneol, camphor and internal standard was 95, 95 and 128, respectively. Linearity, accuracy, precision and extraction recovery of the analytes were all satisfactory. The method was successfully applied to pharmacokinetic studies of NB after oral administration to Wistar rats.
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Index:
WPRIM
Language:
Zh
Journal:
Journal of Pharmaceutical Analysis
Year:
2014
Type:
Article