Effect of IKK16 on proliferation and apoptosis of human glioblastoma cell line U 87 / 重庆医学

ABSTRACT

Objective To investigate the effecof Iκkinases(IKK) inhibitoIKK16 on the proliferation and apoptosiof hu-man glioblastomcell line U87 and itpossible mechanism .MethodHuman glioblastomcell line U87 wacultured in vitro and divided into 4 groupblank group(withouintervention treatmen) ,control group(1% dimethyl sulfoxide) ,low dose IKK group (70 nmol/L IKK16) and high dose IKK group(200 nmol/L IKK16) .The Mtassay waused to detecthe effecof IKK16 on the proliferation of U87 cellathe low concentration and the high concentration .Western blowaused to investigate the effecof IKK16 on the expression of p65 ,cyclinD1 ,caspase-3 and bcl-2 .ResultThe U87 proliferation rate had no statistical difference be-tween the blank group and the control group(P＞0 .05) .Compared with the control group ,IKK16 could significantly suppresthe proliferation of U87 cell(P＜0 .05) .Athe same time ,IKK16 significantly reduced the expression of p65 ,CyclinD1 and Bcl-2 ,and increased the expression of caspase-3(P＜0 .05) with dose-dependenmanne.Conclusion IKK16 could suppresthe proliferation of human glioblastomcell line U87 and promote the apoptosi.