Generation of induced pluripotent stem cells and neural cells from urine-derived cells of Alzheimer disease patients / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12): 421-427, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-474081
ABSTRACT
[ ABSTRACT] AIM:
In this study, we aim to obtain the induced pluripotent stem cells ( iPSCs) from the patients with sporadic Alzheimer disease ( AD) .METHODS:
Three typical Alzheimer’ s patients were chosen, and the epithelial cells were isolated from their urine.We reprogrammed these cells into induced pluripotent stem cells by transfection of 4 factors (Oct4, Sox2, Klf4 and SV40LT) with the technique of electro-transfection.After getting these iPSCs, we continue to differentiate them into neural cells by a specific method—dual inhibition of Smad signaling.RESULTS:
The primary cells from 3 AD patients were successfully reprogrammed to iPSCs, and these patients-derived iPSCs were differentiated into neural cells.There was no significant difference, during iPSCs reprogramming and neural differentiation, between cells from AD patients and normal people.CONCLUSION:
The urine cells from AD patients were able to transfer to iPSCs, functional neurons and neurogliocytes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
2015
Type:
Article
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