Modified platelet-rich plasma promotes the osteogenic differentiation of stem cells from human exfoliated deciduous teeth / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6): 805-808, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-475208
ABSTRACT
Objective:
To investigate the effect of modified plateletrich plasma(mPRP)on the osteogenic differentiation of stemcells from human exfoliated deciduous teeth(SHED).Methods:
mPRP at 1%,2%,5%,10% and FBS at 10% were added to thecultured SHED of passage 4,respectively.The influence of mPRP on alkaline phosphatase(ALP)activity was evaluated using ALPkit.RUNX2 and osteocalcin mRNA expression in the treated cells were examined by realtime PCR.Results:
mPRP enhanced ALPactivity in the SHED,and the effect of mPRP was more obvious at 2%.Treatment of the cells with 2% mPRP upregulated the mRNAexpressions of RUNX2 and osteocalcin.Conclusion:
mPRP can promote the osteogenic differentiation of SHED.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Practical Stomatology
Year:
2014
Type:
Article
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