Your browser doesn't support javascript.
loading
Expression of matrix metalloproteinases-9 in the brain tissue of experimental hepatic encephalopathy rats / 中国基层医药
Chinese Journal of Primary Medicine and Pharmacy ; (12): 175-177, 2015.
Article in Chinese | WPRIM | ID: wpr-475498
ABSTRACT
Objective To investigate the significance of MMP-9 expression in the pathogenesis of hepatic encephalopathy.Methods 180 rats were randomly divided into the control group,hepatic encephalopathy 4,8 and 12h groups,45 rats in each group.In hepatic encephalopathy group,rats were intraperitoneally injected D-galactosamine (400mg/kg) with lipopolysaccharide (100μg/kg),and then the symptom was observed.Ammonia,the evans blue(EB) content,the brain water content and the MMP-9 mRNA in the brain tissue were assessed by real-time Q-PCR after 4,8 and 12h.Results 2h later the rats in hepatic encephalopathy groups began to appear the symptom.4 hours later,ammonia,the EB content,the brain water content and MMP-9 mRNA content of the hepatic encephalopathy groups began to increase [(74.27 ± 1.77) μ mol/L vs (33.85 ± 1.50) μ mol/L,F =5 983.36 ;(4.84 ±0.43)μg/g vs (3.96 ±0.48)μg/g,F =111.54; (78.63 ± 0.46)% vs (76.07 ±0.39)%,F =737.25,and (1.037 ± 0.071) vs (0.829 ± 0.053),F =132.10,P < 0.01],and with the extension of time increased obviously [(136.32 ± 3.88) μmol/L and (279.53 ± 9.78) μmol/L,F =5 983.36 ; (5.94 ± 0.35) μg/g and (6.71 ±0.50)μg/g,F=111.54;(80.50±0.32)% and (82.14±0.30)%,F=737.25;(1.235 ±0.095) and (1.425± 0.113),F =132.10,P < 0.01].Conclusion Blood brain barrier permeability enhancement and brain edema in hepatic encephalopathy rats may be related with MMP-9 mRNA increasing.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Primary Medicine and Pharmacy Year: 2015 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Primary Medicine and Pharmacy Year: 2015 Type: Article