Determination of AsperosaponinⅥ in Dieda Cuyu Tablets by HPLC / 中国药师
China Pharmacist
;
(12): 1770-1772, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-475740
ABSTRACT
Objective:
To establish a method for the determination of asperosaponinⅥin Dieda Cuyu tablets by HPLC.Methods:
A Hypersil C18(250 mm ×4.6 mm,5 μm)column was used. The mobile phase was acetonitrile-water(30∶70) with a flow rate of 1.0 ml·min-1 . The detection wavelength was 212 nm, the column temperature was room temperature,and the injection volume was 10μl.Results:
AsperosaponinⅥ showed a good linear relationship within the range of 0. 04-0. 32 μg(r=0. 999 6). The average recovery was 97. 84%(RSD=1. 70%, n=6).Conclusion:
The method is simple,accurate and reproducible, which can be used in the deter-mination of asperosaponinⅥ in Dieda Cuyu tablets.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2014
Type:
Article
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