Establishment and Application of TaqMan Real-Time PCR assay for Diagnosis of Streptococcus Pneumoniae / 现代检验医学杂志
Journal of Modern Laboratory Medicine
;
(4): 60-63, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-475981
ABSTRACT
Objective To develop TaqMan real-time PCR assay for detection and identification of streptococcus pneumonia iso-lated from children CAP.Methods Based on the sequences of lytA gene,primers and probe were designed and the assay was optimized.Then 1 504 sputum samples were detected by culture and the developed assay with double-blind testing.Results The lower limit of detection in the developed real-time PCR assay was 18.75 cfu/PCR,and had no cross reaction.141 strep-tococcus pneumonia strains were detected from 1 504 samples and 140 were isolated by culture.The whole process just nee-ded 2.5 h.Conclusion The established assay is rapid,simple,high sensitivity and specificity.It is not only valuable for the i-dentification of streptococcus pneumonia,but also provide evidence for antibiotic therapy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Controlled clinical trial
/
Diagnostic study
Language:
Chinese
Journal:
Journal of Modern Laboratory Medicine
Year:
2014
Type:
Article
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