Influence of Vaginal Discharge on Dry Chemistry Determination of Leukocyte Esterase in Female Urine / 现代检验医学杂志

ABSTRACT

Objective The present study is to investigate the influence of vaginal discharge on dry chemistry determination of leukocyte esterase in female urine.Methods Collected 20 and 30 normal vaginal discharge samples.Which humoral routine test degree were Ⅰ and Ⅱ correspondingly,and then analyze leukocyte esterase and squamous epithelial cells in these normal vaginal discharge samples.Collected normal vaginal discharge samples again and isolate the vaginal squamous epithelial cells from those normal vaginal discharge samples.Made two concentration of squamous epithelial cell suspension liquid (20/μl and 60/μl)and added these liquid.To normal female urine and analyze the drying chemical leukocyte esterase in those urine. Cleaned the vulva of those patients with normal leukocyte counts in urine sediment determination,whose dry chemical deter-mination of leukocyte esterase was strong positive,perform the routine urine test with her middle urine.Results The results of the determination of leukocyte esterase in normal vaginal discharge samples were 66.7% positive.And there were ~squamous epithelial cells in all samples (microscopy).No significant difference was observed in the examination of dry chem-istry leukocyte esterase among the normal female urine group,low (20/μl)and high (60/μl)concentrations of squamous epi-thelial cell urine group (P >0.05).The counts of squamous epithelial cells and the rate of positive and intensity of dry chem-ical leukocyte esterase in the middle of second urine were significantly lower than before,and the differences are statistically significant (P <0.05).But there was no significant difference for leukocytes counts.Conclusion Urine squamous epithelial cells had no influence on the detection of leukocyte esterase by dry chemistry.However,the leukocyte esterase in the vaginal discharge greatly influenced the examination of the leukocyte esterase in urine.