Protective effect and mechanism of parthenolide in rats with doxorubicin-induced nephropathy / 中国生化药物杂志

ABSTRACT

Objective To observe protective effects of parthenolide ( PTN) in rats with doxorubicin-induced nephropathy and its mechanims. Methods 48 male rats were randomly divided into six groups blank group, model group, vehicle group, and parthenolide-treated group (1.5, 3, 6 mg/kg).The models of doxorubicin-induced nephropathy were established by tail vein injection of 5 mg/kg doxorubicin for one time.After consecutive treatment of PTN for seven days, the BUN and Cr in serum, and nitricoxide (NO), malondialdehyde (MDA), nitricoxidesynthase (NOS), glutathione peroxidase (GSH), superoxide dismutase (SOD) in renal tissues were detected.The pathological changes of renal tissue was observed by HE method. ResuIts There was significant pathological changes of renal tissue in model group, that showed basophilic change, interstitial inflammatory hyperplasia and inflammatory infiltratio, after PTN 6 mg/kg treatment, the above changes significant improved.The serum BUN and Cr levels in PTN 1.5 mg/kg, PTN 3 mg/kg and PTN 6 mg/kg group were lower than those in model group (P<0.05);there were no significant differences of above indexes between PTN 3 mg/kg, PTN 6 mg/kg group and blank gorup.Compared with model group, the SOD and GSH activity were higher and MDA level was lower in three different dosage of PTN groups (P<0.05); there were no significant differences of SOD activity between PTN 3 mg/kg, PTN 6 mg/kg group and blank gorup; there were no significant differences of GSH activity and MDA level between PTN 6 mg/kg group and blank gorup.The NO level and NOS activity in three different dosage of PTN groups were higher (P<0.05);there were no significant differences of NO level and NOS activity between PTN 6 mg/kg group and blank gorup.ConcIusion Parthenolide has protective effects on doxorubicin-induced nephropathy, and its mechanism might be antioxidative effects.