Osteogenesis of adipose stem cells on artificial bone scaffold / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 4493-4497, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-476848
ABSTRACT
BACKGROUND:
Insufficient source of seed cel s is the important factor to restrict the tissue reconstruction and the development of regenerative medicine.OBJECTIVE:
To explore the osteogenesis of adipose stem cel s cultured with different kinds of artificial bones.METHODS:
Adipose tissue was extracted from female volunteers undergoing cosmetic surgery to isolate adipose stem cel s. Passage 4 adipose-derived mesenchymal stem cel s were selected and divided into osteogenic induction group, osteogenic induction+hydroxyapatite group, osteogenic induction+absorbable bone group and osteogenic induction+recombinant bone xenograft group. The latter three groups were subdivided into 3, 10, 20 g/L subgroups, respectively. Culture medium was exchanged every 3 days, total y for 12 days. RESULTS ANDCONCLUSION:
Compared with the osteogenic induction group, the calcium concentration in the elution liquid was significantly higher in the osteogenic induction+hydroxyapatite group and low-concentration osteogenic induction+absorbable bone group (both P<0.05), but no difference was found between the osteogenic induction group and high-concentration osteogenic induction+absorbable bone group (P<0.05). In addition, the calcium concentration in the elution liquid was significantly lower in the osteogenic induction+recombinant bone xenograft group than the osteogenic induction (P<0.05). Therefore, different artificial bone scaffolds can influence the osteogenic effect of adipose stem cel s, and among them, hydroxyapatite has a better effect on the osteogenic induction of adipose stem cel s.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Tissue Engineering Research
Year:
2015
Type:
Article
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