Biocompatibility of bio-derived xenograft bone with bone marrow mesenchymal stem cells / 中国组织工程研究

ABSTRACT

BACKGROUND:Bio-derived xenograft bone has natural pore structure of the bone, low immunogenicity, and good cytocompatibility. OBJECTIVE:To verify the biocompatibility of bio-derived xenograft bone with bone marrow mesenchymal stem cel s. METHODS:Fresh pig femoral bone was col ected to prepare bio-derived bone. Scanning electron microscope was used to observe the material structure. Passage 3 rabbit bone marrow mesenchymal stem cel s at a density of 2×109/L were inoculated into the cancel ous bone surface of the bio-dervied bone and cultured for 7 days. Cel growth was detected using scanning electron microscope. After culture for 8 days, cel number was counted. RESULTS AND CONCLUSION:The bio-derived bone had rough surface and irregularly interconnected pores constructing a mesh-like structure. After 3 days of compound culture, cel s had irregular shapes and adhered to the surface of bio-derived bone;after 5 days of culture, cel s were closely interconnected to form a layered growth;after 7 days of culture, cel s exhibited multilayered growth and the extracel ular matrix secreted local y. Under compound culture, the former 2 days were latent period, 3-6 days were logarithmic phase, and from the 6 th day, the cel growth curve became smooth gradual y and the cel proliferation decreased and entered into the plateau period. These findings indicate that the bio-derived xenograft bone has good biocompatibility with bone marrow mesenchymal stem cel s.