Study on cell counting random error of the practical McAb-A-E direct method for cell sheet of T lymphocyte subsets / 国际检验医学杂志

ABSTRACT

Objective To research and evaluate cell counting random error for cell sheet of T lymphocyte subsets in the practical McAb‐A‐E direct method ,and old method of McAb‐A‐E direct method compared .Methods Randomly selected 20 specimens ,cell sheet of T lymphocyte subsets on counting 200 cell at least 2 area from 2/6~4/6 area ,counting area non‐repeated ,CD4+ and CD8+continuous counter 4 times ,CD3+ continuous counter 2 times .Results In the practical McAb‐A‐E direct method ,random error mean of cell counting was separately CD3+7 .86 ,CD4+9 .99 ,CD8+8 .55 ,CD4+ /CD8+0 .33 ,lower than old method of McAb‐A‐E direct method ,that was CD3+15 .00 ,CD4+13 .80 ,CD8+10 .70 ,CD4+ /CD8+0 .69 .Conclusion Cell counting random error in practical McAb‐A‐E direct method for cell sheet of T lymphocyte subsets is less than old method of McAb‐A‐E direct method .