Clinical significance of microRNA-7 expression and its correlation with focal adhesion kinase in colorectal cancer / 中华消化杂志

ABSTRACT

Objective To investigate the expression and clinical significance of microRNA‐7 (miRNA‐7) and focal adhesion kinase (FAK) in colorectal cancer (CRC) .Methods Sixty pairs of CRC and adjacent colorectal tissues were collected .The expression of FAK was detected by immunohistochemistry and the expression of miRNA‐7 was determined by quantitative real‐time polymerase chain reaction (PCR) . Chi square test was used for statistical analysis and Spearman rank was applied for correlation analysis . Results The positive rate of FAK expression in CRC was 75 .0% (45/60) and that in adjacent colorectal tissues was 26 .7% (16/60) ,the difference was statistically significant (χ2 = 28 .04 , P < 0 .01) . The positive rate of phospho‐FAK (p‐FAK ) expression in CRC was 65 .0% (39/60 ) and that in adjacent colorectal tissues was 21 .7% (13/60) ,and the difference was statistically significant (χ2 = 22 .94 , P <0 .01) .The expression of miRNA‐7 in CRC tissues was down‐regulated compared with that in adjacent colorectal tissues (P= 0 .044) .The correlation between miRNA‐7 expression and lymph nodes metastasis was negative in patients with CRC (Z= - 2 .290 ,P= 0 .022) .The miRNA‐7 expression was significantly negatively correlated with TNM stage in patients with CRC (Z= - 2 .698 , P= 0 .007) .However it was not correlated with age ,gender ,the location of tumor and tumor differentiation .The correlation between miRNA‐7 expression and FAK ,p‐FAK was negative (Z= - 0 .303 ,P= 0 .019 ;Z= - 0 .267 ,P= 0 .038) . Conclusion The miRNA‐7 may involved in the genesis and development of CRC through regulating the expression of FAK ,which provide a new target for the diagnosis and treatment of CRC .