Effects of Radix Paeoniae Rubra Extracts on the Endoplasmic Reticulum Stress and Macrophage Recruitment of Diabetic Cardiomyopathy Rats / 医药导报

ABSTRACT

Objective To investigate the protective effects of Radix Paeoniae Rubra(RPR)extracts on reticulum stress and macrophage infiltration on diabetic cardiomyopathy rats and the mechanism. Methods Sprague￣Dawley rats were randomly divided into 5 groups: normol control, model control (induced by streptozotocin),RPR extracts at low dose (i.p.15 g.kg-1 ), middle dose (i.p.30 g.kg-1 ), and high dose (i.p.60 g.kg-1 ).The artery intubation was carried out in all rats for detecting cardiac function 10 weeks later.The myocardial structure was detected by masson staining.The level of myocardial tissue glucose regulatory proteins GRP78,caspase￣12 and caspase￣3 expression were measured by Western blot.The ED￣1 protein was tested by immunohistochemical staining. Results Compared with the normal controls, the model rats presented decreased LVSP, +dp/dtmax ,-dp/ dtmax , LVEF, increased expressions of GRP78, caspase￣12,caspase￣3, ED￣1 and the synthesis of collagen fiber(P<0.05 or P<0.01).RPR extract inhibited the above indexes of model control rats in a concentration￣dependent manner(P<0.05 or P<0.01). Conclusion The RPR obviously ameliorates the cardiac function and myocardial fibrosis of diabetic cardiomyopathy rats,which may be associated with suppressing the expression of GRP78,caspase￣12,caspase￣3 and macrophage infiltration.