Celastrol induces caspase-dependent apoptosis through ROS/JNK path-way in Saos-2 cells / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12): 1457-1461, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-477354
ABSTRACT
AIM:
ToexploretheeffectofcelastrolonapoptosisofSaos-2cellsanditsmechanism.METH-ODSSaos-2 cells were treated with various concentrations of celastrol , and the cell viability was measured by MTT assay . Apoptosis and reactive oxygen species ( ROS) production were determined by flow cytometry .The protein levels of cleaved caspase-9, cleaved caspase-3 and phosphorylated JNK were evaluated by Western blot .RESULTS:
The viability of Saos-2 cells was significantly inhibited by celastrol .Celastrol significantly induced apoptosis of Saos-2 cells.Celastrol signifi-cantly induced ROS production in the Saos-2 cells.Western blot analysis demonstrated that celastrol significantly increased the protein levels of cleaved caspase-9, cleaved caspase-3 and phosphorylated JNK in the Saos-2 cells.CONCLUSION:
Celastrol induces caspase-dependent apoptosis through ROS/JNK pathway in Saos-2 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
2015
Type:
Article
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