Usefulness of PCR-SSP Method for Detection of HLA-DRB1*0405 / 임상검사와정도관리
Journal of Laboratory Medicine and Quality Assurance
;
: 201-205, 2007.
Article
in Korean
| WPRIM
| ID: wpr-47771
ABSTRACT
BACKGROUND:
The association between HLA-DRB1 gene and severity of rheumatoid arthritis (RA) has been documented in various reports. Especially, DRB1*0405 allele shows significant association with RA in Korean patients. DRB1*0405 typing has been performed by the sequence based typing method (SBT), that is a difficult and expensive method. So we tried to replace it with a simple and inexpensive method.METHODS:
We performed the HLA-DRB1*0405 typing using PCR-SSP technique with sequence specific primers of 3 pair in 298 Koreans. These results were compared with those of high resolution sequence based typing (SBT) method.RESULTS:
Of 298 samples typed with the high resolution SBT method, 60 samples were HLA-DRB1*04 positive and showed 9 subgroups of HLA-DRB1*04 and 26 samples were HLA- DRB1*0405 positive. With the PCR-SSP method, same 26 samples were HLA-DRB1*0405 positive, showing 100% correspondence between two methods to detect HLA-DRB1*0405CONCLUSIONS:
Using PCR-SSP method to type HLA-DRB1*0405 is a very useful tools for studying the association between rheumatoid arthritis and HLA-DRB1*0405 from a practical and economic view.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Arthritis, Rheumatoid
/
HLA-DR Antigens
/
Alleles
/
HLA-DRB1 Chains
Type of study:
Diagnostic study
Limits:
Humans
Language:
Korean
Journal:
Journal of Laboratory Medicine and Quality Assurance
Year:
2007
Type:
Article
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