Evaluation of EuDx™-PN MLC Detection Kit for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella pneumophila in Respiratory Specimens / 대한임상미생물학회지
Annals of Clinical Microbiology
;
: 97-102, 2017.
Article
in Korean
| WPRIM
| ID: wpr-47832
ABSTRACT
BACKGROUND:
Infection by the intracellular bacteria Mycoplasma pneumoniae, Chamydophila pneumoniae, and Legionella pneumophila are common causes of community-acquired pneumonia (CAP). This study describes the evaluation of a new multiplex real-time PCR test, EuDx™-PN MLC Detection Kit (EUDIPIA), which allows the simultaneous detection of M. pneumoniae, C. pneumoniae, and L. pneumophila in respiratory samples.METHODS:
A total of 353 samples were tested using three PCR kits multiplex PCR (Seeplex PneumoBacter ACE Detection Kit) and two multiplex real-time PCR (EuDx™-PN MLC Detection Kit and Anyplex™ II RB5 Detection Kit). The results were considered true positives (expanded standard) for M. pneumoniae, C. pneumoniae, and L. pneumophila if they were positive according to any of the three tests.RESULTS:
The sensitivity and specificity of EuDx™-PN MLC Detection Kit were 93.3–100% and 100%, respectively. The agreement rate and Cohen's kappa coefficient (value) between EuDx™-PN MLC Detection Kit and Anyplex™ II RB5 Detection Kit for M. pneumoniae, C. pneumoniae, and L. pneumophila were 70–100% and 0.82–1, respectively.CONCLUSION:
These results demonstrate that the EuDx™-PN MLC Detection Kit is a sensitive, specific, and useful screening tool for the detection of atypical pathogens in respiratory samples and can be helpful in selecting appropriate antimicrobial therapy for patients with respiratory infection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pneumonia
/
Pneumonia, Mycoplasma
/
Respiratory Tract Infections
/
Bacteria
/
Legionella
/
Mass Screening
/
Polymerase Chain Reaction
/
Sensitivity and Specificity
/
Legionella pneumophila
/
Chlamydophila pneumoniae
Type of study:
Diagnostic study
/
Screening study
Limits:
Humans
Language:
Korean
Journal:
Annals of Clinical Microbiology
Year:
2017
Type:
Article
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