The cytotoxicity of injectable PHEMA/CPC composite hydrogel and it's effect on cell apoptosis / 实用口腔医学杂志

ABSTRACT

Objective:To study the cytotoxicity of PHEMA /CPC composite hydrogel.Methods:L929 cells were cultured by RP-MI1 640 with 1 0% fetal bovine serum(blank control),with PHEMA /CPC extraction(experimental group),high density polyethy-lene(HDPE)extracts(negative control)and 5% DMSO(positive control)for 24,48 and 72 h respectively.The cell proliferation was examined by MTT assay.Cell apoptosis was detected by flow cytometry and Annexin V-FITC /PI kit.Results:The A value of positive control group decreased with the increase of culture time(P 0.05).The cytotoxicity of the experimental group was grade Ⅰ.That of other groups increased with the increase of culture time. Cell apoptosis(%)in PHEMA /CPC composite hydrogel group and blank group was 4.21 ±0.30 and 4.89 ±0.39 respectively(P>0.05).Conclusion:Injectable PHEMA /CPC composite hydrogel has no cytotoxicity and no effect on cell apoptosis.