Effect of Electrical Stimulation to Cerebellar Fastigial Nucleus on Cerebral Ischemia-reperfusion Injury in Rats / 中国康复理论与实践

ABSTRACT

Objective To investigate the effect of electrical stimulation to cerebellar fastigial nucleus on expression of nuclear fac-tor-kappa B (NF-кB) P50, tumor necrosis factor-α(TNF-α) and Bcl-xL mRNA in rats brain after cerebral ischemia-reperfusion. Methods Sprague-Dawley rats were randomly divided into normal control group (NC group), cerebral ischemia-reperfusion group (I/R group), fasti-gial nucleus stimulation (FNS) group, and fastigial nucleus lesion (FNL) group. A focal cerebral ischemia-reperfusion model was established with middle cerebral artery occlusion (MCAO). 7 and 14 days after operation, the infarct volume was measured, and the protein of NF-кB P50 in rats brain was detected with Western blotting;the expression of TNF-αand Bcl-xL mRNA was detected with RT-PCR. Results Com-pared with I/R group, the expression of NF-кB P50 protein increased in FNS group (P0.05). Conclusion FNS could induce the expression of P50 protein and Bcl-xL mRNA, and inhibit the expression of TNF-αmRNA, and reduce infarct size, which may associated with the neuroprotection of central ner-vous system from injury.