Calpains are Involved in Entamoeba histolytica-Induced Death of HT-29 Colonic Epithelial Cells
The Korean Journal of Parasitology
;
: 177-180, 2011.
Article
in English
| WPRIM
| ID: wpr-47943
ABSTRACT
Entamoeba histolytica is an enteric tissue-invading protozoan parasite that can cause amebic colitis and liver abscess in humans. E. histolytica has the capability to kill colon epithelial cells in vitro; however, information regarding the role of calpain in colon cell death induced by ameba is limited. In this study, we investigated whether calpains are involved in the E. histolytica-induced cell death of HT-29 colonic epithelial cells. When HT-29 cells were co-incubated with E. histolytica, the propidium iodide stained dead cells markedly increased compared to that in HT-29 cells incubated with medium alone. This pro-death effect induced by ameba was effectively blocked by pretreatment of HT-29 cells with the calpain inhibitor, calpeptin. Moreover, knockdown of m- and micro-calpain by siRNA significantly reduced E. histolytica-induced HT-29 cell death. These results suggest that m- and micro-calpain may be involved in colon epithelial cell death induced by E. histolytica.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Calpain
/
Cell Line
/
Cell Survival
/
Cell Death
/
Dipeptides
/
Entamoeba histolytica
/
Epithelial Cells
/
Gene Knockdown Techniques
Limits:
Humans
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2011
Type:
Article
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