Endogenous Gastric-Resident Mesenchymal Stem Cells Contribute to Formation of Cancer Stroma and Progression of Gastric Cancer
Korean Journal of Pathology
;
: 507-518, 2013.
Article
in English
| WPRIM
| ID: wpr-47968
ABSTRACT
BACKGROUND:
Carcinoma-associated fibroblasts (CAFs) contribute to carcinogenesis and cancer progression, although their origin and role remain unclear. We recently identified and investigated the in situ identity and implications of gastric submucosa-resident mesenchymal stem cells (GS-MSCs) in the progression of gastric carcinogenesis.METHODS:
We isolated GS-MSCs from gastric submucosa using hydrogel-supported organ culture and defined their identity. Isolated cells were assessed in vitro by immunophenotype and mesengenic multipotency. Reciprocal interactions between GS-MSCs and gastric cancer cells were evaluated. To determine the role of GS-MSCs, xenografts were constructed of gastric cancer cells admixed with or without GS-MSCs.RESULTS:
Isolated cells fulfilled MSCs requirements in regard to plastic adherence, stromal cell immunophenotype, and multipotency. We demonstrated a paracrine loop that gastric cancer cells enhanced the migration, proliferation, and differentiation of GS-MSCs; additionally, GS-MSCs promoted the proliferation of gastric cancer cell in vitro. Xenograft experiments showed that GS-MSCs significantly promoted cancer growth and angiogenesis. GS-MSCs that integrated into gastric cancer became not only CAFs but also rarely endothelial cells which contributed to the formation of cellular and vascular cancer stroma.CONCLUSIONS:
Endogenous GS-MSCs play an important role in gastric cancer progression.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organ Culture Techniques
/
Plastics
/
Stomach Neoplasms
/
Transplantation, Heterologous
/
Stromal Cells
/
Endothelial Cells
/
Mesenchymal Stem Cells
/
Fibroblasts
/
Heterografts
/
Carcinogenesis
Language:
English
Journal:
Korean Journal of Pathology
Year:
2013
Type:
Article
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