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A new sandwich ELISA method for quantitative analysis of fusion protein IL-2-HSA / 中国药科大学学报
Journal of China Pharmaceutical University ; (6): 175-179, 2010.
Article in Chinese | WPRIM | ID: wpr-480364
ABSTRACT
A double antibody sandwich ELISA for quantitative analysis of recombinant fusion protein IL-2-HSA was constructed using a polyclonal antibody to human IL-2 for capture and a monoclonal antibody to HSA with HRP-labeled conjugate for detection.The optimal concentration of the first coating antibody and detection antibody were 2 μg/mL and 0.5 μg/mL,respectively.Regression equation of the linear calibration curve wasy = 0.442 9 x-1.143 3 with a correlation coefficient of 0.996 6,and the linear detection ranged from 39.06 ng/mL to 1 250 ng/mL.Recovery from the supernatant of fermentation broth was 98.13% to 102.94%.The specificity assay indicated that it had little cross-reactions with IL-2 and HSA.The soundness analysis suggested that fermentation broth,mouse serum and dilution had no influence on the method.The present method can be used in the studies on fermentation,purification and clinical diagnosis.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of China Pharmaceutical University Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of China Pharmaceutical University Year: 2010 Type: Article