RP-HPLC gradient elution analysis for the determination of pantoprazole soldium and its related substances / 中国药科大学学报
Journal of China Pharmaceutical University
;
(6): 531-534, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-480416
ABSTRACT
Aim:
To establish a method for the determination of pantoprazole sodium and its related substances.Methods:
A column packed with octadecylsiance bonded silica gel (250 mm × 4. 6 mm, 5 pjn) was used. The 0. 01 mol/L monopotassium phosphate solution( adjusted with phosphoric acid to pH 7. 0) -acetonitrile were adopted as the mobile phase, a gradient elution was programmed as follows 0→30 min(9010-6040), 30→45 min(60 40→15 85); the detection wavelength was 289 nm; the column temperature was 40 ℃; the flow rate was 1. 0 mL/min.Results:
Pantoprazole sodium, the intermediates and its related substances could be well separated. A good linear relationship was obtained over the range of 6. 96-48. 72 μg/mL( r =0. 999 9). The limit detection and quantisation of pantoprazole sodium were 8.51 ng and 17.0 ng, respectively.Conclusion:
This method can be applied to control the related substances of pantoprazole sodium and determine pantoprazole sodi-um.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of China Pharmaceutical University
Year:
2009
Type:
Article
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