Regenerated cellulose materials for skin repair：preparation and performance / 中国组织工程研究

ABSTRACT

BACKGROUND:The regenerated celulose membrane has good moisture absorption and mechanical strength as wel as good biodegradability, but its toughness is poorer. Studies have shown that the dry film of regenerated celulose membrane has a poor elongation rate that is less than 15%. OBJECTIVE: To prepare the compound regenerated celulose membrane/colagen membrane, and to test its mechanical properties, sweling properties, moisture permeability and water retention. METHODS:Using sodium periodate oxidation method, the 2,3-dialdehydecelulose/colagen membrane was prepared. Infrared spectroscopy and scanning electron microscope were used to observe the morphological structure, and test its mechanical properties, sweling properties, moisture permeability and water retention. Meanwhile, the regenerated celulose membrane and 2,3-dialdehydecelulose membrane served as controls. Fibroblasts from logarithmic phase mouse embryos were seeded onto the 2,3-dialdehydecelulose/colagen membrane, and after 1, 3, 5 days, MTT method was used to detect cel proliferation. RESULTS AND CONCLUSION:Infrared spectra showed that the colagen was cured to the surface of 2,3-dialdehydecelulose membrane. Under the scanning electron microscopy, the 2,3-dialdehydecelulose/ colagen membrane had loose porous structure, with a porosity of 93.4%. The mechanical properties, sweling rate and moisture permeability of the 2,3-dialdehydecelulose/colagen membrane were significantly stronger than those of the 2,3-dialdehydecelulose membrane and regenerated celulose membrane (P < 0.05). However, there was no difference in the water retention rate among the three groups. Mouse embryonic fibroblasts tightly adhered to the surface of regenerated celulose/colagen membrane, and presented a proliferative trend. These findings indicate that the 2,3-dialdehydecelulose/colagen membrane has good mechanical properties, sweling property, moisture permeability, water retention, and cytocompatibility.