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Uroplakin mRNA Expression in the Micro-dissected Mouse Prostate / 대한남성과학회지
Korean Journal of Andrology ; : 112-117, 2010.
Article in Korean | WPRIM | ID: wpr-48078
ABSTRACT

PURPOSE:

Prostatitis is a common condition with a significant effect on quality of life. Even though the etiology of chronic prostatitis remains unclear, certain bacterial infections may play a major role. In recent studies, E. coli, one important etiology of urinary tract infection, was found to mediate invasion into the bladder epithelium after binding uroplakin Ia in the apical membrane of the urinary bladder. Because E. coli is also an important pathogen for bacterial prostatitis, we investigated the uroplakin mRNA expression in micro-dissected mouse prostates. MATERIALS AND

METHODS:

We harvested the urinary bladder, ventral prostate, dorso-lateral prostate, and coagulating gland from 3 male imprinting control region (ICR) mice. The total RNA was extracted, cDNA was prepared, and finally the five target genes--uroplakin Ia, Ib, II, III, and beta-actin were amplified. We also examined the expressed sequence tags (EST) about above four uroplakin genes from mouse EST data.

RESULTS:

Uroplakin Ia, Ib, II, and III were expressed in the urinary bladder. However, only uroplakin Ia was definitively expressed in the ventral prostate. Uroplakin Ib and II were weakly expressed in the ventral, dorso-lateral, and coagulating prostate. Uroplakin III was not expressed in the prostate tissue. The mouse RNA transcripts in the EST data also showed similar results to uroplakin expression in the prostate.

CONCLUSIONS:

These results suggest that the mouse ventral prostate may be an adequate locus for acute or chronic bacterial prostatitis study. Further in-vitro bacteriologic studies of the ventral prostate will help reveal the mechanisms of chronic bacterial prostatitis.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Prostate / Prostatitis / Quality of Life / Bacterial Infections / Urinary Tract Infections / Urinary Bladder / RNA / RNA, Messenger / Actins / DNA, Complementary Limits: Animals / Humans / Male Language: Korean Journal: Korean Journal of Andrology Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Prostate / Prostatitis / Quality of Life / Bacterial Infections / Urinary Tract Infections / Urinary Bladder / RNA / RNA, Messenger / Actins / DNA, Complementary Limits: Animals / Humans / Male Language: Korean Journal: Korean Journal of Andrology Year: 2010 Type: Article