Expressions of MCM5 and P16INK4A in cervical squamous cell carcinoma / 西安交通大学学报(医学版)

ABSTRACT

Objective To investigate the expressions of minichromosome maintenance protein 5 (MCM5)and P1 6 INK4A gene and protein in cervical squamous cell cancer (SCC),cervical intraepithelial neoplasia (CIN)and normal tissues.Methods Real-time PCR and immunohistochemistry were used respectively to detect the expression levels of mRNA and protein of MCM5 and P1 6 INK4A in 40 cases of cervical cancer,1 1 cases of CIN I,1 5 cases of CIN Ⅱ - Ⅲ and 1 5 cases of normal cervical tissues.Results ① The expression levels of mRNA and protein of MCM5 and P1 6 INK4A in tissues of cervical squamous cell cancer were significantly higher than those in normal cervical tissues,CIN I and CIN Ⅱ-Ⅲ tissues (P 0.05 ).② The high expression of MCM5 mRNA and protein in cervical squamous cell carcinoma was positively correlated with clinical stage and differentiation grade of cervical cancer (P 0.05).P1 6 INK4A expression exhibited no correlation with clinical stage or age of patients (P >0.05),but positive correlation with differentiation grade (P <0.01).③ A positive correlation was found between MCM5 and P1 6 INK4A expressions in cervical cancer (r = 0.538,P <0.01).Conclusion The over-expressions of MCM5 and P1 6 INK4A may play an important role in the carcinogenesis and progression of cervical cancer.MCM5 may be used as a new marker of the proliferation of cervical cancer. Detection of P1 6 INK4A and MCM5 is of great significance to studying the grade and outcome of CIN,which may improve the rate of cervical cancer screening.