Determination of Naringenin and Apigenin in Premna fulva Craib.by HPLC / 医药导报
Herald of Medicine
;
(12): 1483-1485, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-481295
ABSTRACT
Objective To establish a HPLC method for determination of naringenin and apigenin in Premna fulva. Methods The SHISEIDO ̄SPOLAR C18(250 mm×4.6 mm,5μm) was used as analytical column.The mobile phase consisted of methanol ̄0.2% phosphoric acid (4258) with isocratic elution at a flow rate of 1.0 mL.min-1 .The detection wavelength of naringenin and apigenin was 288 nm and 340 nm, respectively.Column temperature was set at 35 ℃ , the injection Volume was 10 μL. Results Naringenin and apigenin had a good linear relationship in the concentration range of 0.180 ~ 3.60 μg (r =0.999 9) and 0. 0052 ~ 0. 1040 μg ( r = 0. 999 9), respectively. Conclusion The method is accurate and reliable. It is appropriate for the quantitative determination of naringenin and apigenin in Premna fulva and its preparations.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Herald of Medicine
Year:
2015
Type:
Article
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