Dickkopf-1 inhibits vasculogenic mimicry formation of colon can-cer and relevant mechanism / 中国肿瘤临床
Chinese Journal of Clinical Oncology
;
(24): 1007-1011, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-481317
ABSTRACT
Objective:
To investigate the inhibitory effect of Dickkopf-1 (Dkk1) on vasculogenic mimicry (VM) formation and the relevant mechanism.Methods:
CD34-PAS dual staining and immunohistochemical staining were used to detect and analyze the re-lationship between VM existence and Dkk1 expression in 217 human colon cancer tissue samples;three dimensional (3D) culture was used to detect the influence of Dkk1 on tube structure formation and on VE-cadherin expression;a subcutaneous mouse xenograft mod-el was made to further validate the inhibitory role of Dkk1 on VM formation in vivo.Results:
VM-positive samples indicated a lower expression of Dkk1(P<0.05);colon cancer cells with Dkk1 overexpression exhibited a decreased ability to form tube-like structure and a decreased expression of VE-cadherin;Dkk1 inhibited the VM-formation abilities of human colorectal carcinoma cell line xenograft tu-mor tissue.Conclusion:
Dkk1 inhibits the VM formation of colon cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Clinical Oncology
Year:
2015
Type:
Article
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