Transfecting pcDNA3-TRAF4-DM-TRAF improves proliferation of human breast cancer cell MDA-MB-231 / 基础医学与临床

ABSTRACT

Objective_To investigate whether overexpression of TRAF4 in human breast cancer may have impact on its cell proliferation.Methods_This study has two groups, MDA-MB-231 transfected with pcDNA3-TRAF4-DM-TRAF or pcDNA3 .We detected the expression and localization of TRAF4 with immunofluorescence and western blot.We detected the expression of the phosphorylation level of p70S6K and S6 with westernblot.Flow cytometry was used to detecte cell cycle.MTT Assay was used to detected cell reproductive capacity.Results_TRAF4 local-ized in both cytoplasm and nuclei in MDA-MB-231.Nuclear expression of TRAF4 in nuclei was lower than that in cytoplasm (P<0.05).After pcDNA3-TRAF4-DM-TRAF was transfected into the cells, the expression of TRAF4 in nuclei was increased (P<0.05).The phosphorylation level of p70S6K and S6 significantly increaseced (P<0.05,P<0.01).More S phase cells were recorded(P<0.01) by FCM.The cell proliferation was promoted by MTT ( P<0.01) .Conclusions_The expression of TRAF4 in nuclei may play an important role in the cell prolif-eratuion by promoting p70S6K and S6 activation.