Prodifen increased thoracic aorta artery stenosis after balloon angioplasty in rat / 基础医学与临床

ABSTRACT

Objective To observe the effect of Prodifen ( Skf525A) on rat thoracic aorta artery stenosis after bal-loon angioplasty and to discuss the relationship between endogenous Cytochrome P 450/epoxyeicosatrienoic acids (CYP2J2/EETs) system and artery stenosis after vascular injury .Methods Totally 24 Wistar rats were randomly divided into four groups assigned to the following treatmentssham group, I group ( treated by ballon angioplasty for 15 d).Using sham+Skf group (administrated with Skf525A), I+Skf group (administrated with Skf525A, then treated by ballon angioplasty for 15 d).Relative luminal area (RLA) and the intimal proliferation index (IPI) were observed by HE staining;The expression of Cytochrome P450 epoxygenase 2J3 (CYP2J3) mRNA was deter-mined by semi-quatative reverse transcription polymerase chain reaction ( RT-PCR ); the level of 11 ,12-EET was detected by high pressure liquid chromatography ( HPLC) .Results Compared with sham group , RLA significantly decreased , IPI significantly increased , CYP2 J3 mRNA expression and the content of 11 ,12-EET all significantly increased in I group ( P<0.01 ) .Compared with I group , Skf525 A treatment significantly decreased RLA , in-creased IPI, and decreased CYP2J3 mRNA expression and the content of 11,12-EET (P<0.01).Conclusions CYP2J3/EETs system can inhibit stenosis after vascular injury .