Biocompatibility of poly-L-lactic acid, a new synthetic material for skin burn / 中国组织工程研究

ABSTRACT

BACKGROUND:Recent studies have shown that poly-L-lactic acid (PLLA) polymer has good biocompatibility and can be directly involved in human metabolism without any side effects, which is a polymer material that can be used as bioscaffolds. OBJECTIVE:To explore the biocompatibility of PLLA. METHODS:The moisture absorption rate of colagen composite and PLLA was detected. Cel morphology observation was carried to detect the growth state of C3H10T1/2 cels that grew in normal HDMEM medium, HDMEM medium+dimethyl sulfoxide, HDMEM medium+colagen composite extract, HDMEM medium+PLLA extract for 72 hours. MTT kit was used to detect the relative proliferation rate of the C3H10T1/2 cel lines that grew in the above-mentioned media and the toxicity evaluations were based on the material toxicity criteria. Hemolysis degree was measured in rabbit blood samples containing normal saline, distiled water, PLLA extract and colagen composite extract. The alergic reaction and pyrogen reaction in rabbits were observed through injection of normal saline, distiled water, PLLA extract and colagen composite extract viathe ear vein. Colagen composite and PLLA were respectively implanted subcutaneously into the rabbits, and after 4 weeks, the levels of interleukin-10 and interleukin 23 in serum were detected. RESULTS AND CONCLUSION: The moisture absorption rates per unit area and per unit mass of colagen composite material were lower than those of PLLA synthetic materials (P < 0.05). C3H10T1/2 cels grew wel in the PLLA extract, with a higher relative growth rate and lower cytotoxicity (grade I). Compared with the colagen composite materials, the PLLA synthetic materials had a lower hemolysis ratio and less inflammatory reactions (P < 0.05); and there were no alergic reaction and pyrogen reaction. These findings indicate that the PLLA scaffold materials for burned skin have good absorbing fluid performance and biocompatibility.