Galectin-3 Expression and BRAF Mutation in Cases of Cytologically Suspicious Papillary Thyroid Carcinoma
Korean Journal of Pathology
;
: 191-198, 2010.
Article
in Korean
| WPRIM
| ID: wpr-48171
ABSTRACT
BACKGROUND:
Fine needle aspiration, which is known as the most accurate and cost-effective method for diagnosis of thyroid nodule, still may result in indeterminate cases that are pauci-cellular and show minor nuclear atypia, but most cases are associated with suspicion of papillary thyroid carcinoma (PTC). A B-type Raf kinase (BRAF) mutation was found in about half of PTCs and galectin-3 was expressed by malignant tumors, helping us to differentiate malignancies from benign lesions.METHODS:
Cases studied included histologically 44 confirmed PTC cases and 18 benign cases previously diagnosed as suspicious of PTC using cytologic examination. Cases were analyzed for galectin-3 expression by immunohistochemical staining and BRAF mutation by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with a new restriction enzyme.RESULTS:
All 44 cases of PTC and 8 of 18 benign controls expressed galectin-3. BRAF mutations were found in only 9 of the 44 PTC cases. Assessment of galectin-3 expression demonstrated high sensitivity but low specificity. Evaluation of BRAF mutation revealed high specificity and low sensitivity.CONCLUSIONS:
This study suggests that the combined application of these two methods for PTC of suspicious cytology is complementary.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphotransferases
/
Thyroid Gland
/
Factor IX
/
Thyroid Neoplasms
/
Carcinoma
/
Sensitivity and Specificity
/
Thyroid Nodule
/
Galectin 3
/
Biopsy, Fine-Needle
Type of study:
Diagnostic study
Language:
Korean
Journal:
Korean Journal of Pathology
Year:
2010
Type:
Article
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