Effect of LX A4 and BML-111 on TLR4/NF-κB signaling pathway in macrophage / 实用医学杂志
The Journal of Practical Medicine
;
(24): 2799-2802, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-481869
ABSTRACT
Objective To determine the effect of lipoxins (LX) A4 and its agonist (BML-111) on the survival of RAW264.7 macrophage cells and TLR4/NF-κB signaling pathway. Methods RAW264.7 cells were treated with different concentrations of LPS, then the effect of LX A4 and BML-111 on the survival rate of these cells was observed. Cytotoxicity were detected by CCK-8 method and RT-PCR was used to detect the TLR4 and TRAF6 mRNA. The protein levels of TLR4 and pNF-κB p65 in RAW264.7 were determined by Western Blot. Results The survival rates of macrophage treated with LPS for 6 h in 1 000 ng/mL LPS in LX A4 group and BML-111 group were significantly higher than that in control group (P 0.05). Conclusion LX A4 and BML-111 could inhibit the cytotoxicity of LPS on the RAW264.7 macrophage cells through the inhibition of the activation of TLR4/NF-κB signaling pathway, then reduce the inflammation. And this stable BML-111 may appear as another promising treatment for IBD disease.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
The Journal of Practical Medicine
Year:
2015
Type:
Article
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