Role of eNOS/NO signaling pathway in peritubular capillary lesions in renal interstitial fibrosis and the related mechanism in mouse models of unilateral ureteral obstruction / 中国实验动物学报

ABSTRACT

Objective To investigate the role of eNOS/NO signaling pathway in peritubular capillary lesions of mouse renal interstitial fibrosis with unilateral ureteral obstruction ( UUC) and the potential mechanism .Methods Sixty-four healthy male KM mice were randomly divided into sham operated group ( n=32 ) and unilateral ureteral obstruction group (n =32).At each week, serum BUN, Scr and NO were determined and the percentages of CD 133 +/VEGFR+en-dothelial progenitor cells in peripheral blood mononuclear cells were detected by flow cytometry .Morphological changes of the renal tissue were observed using Masson staining .The expression of CD34 +cells in the renal interstitium was analyzed by immunohistochemistry to calculate the peritubular capillary density .The expressions of eNos and VEGF mRNA were de-termined by real-time PCR.Results The expression of blood NO , the percentages of endothelial progenitor cells , peritu-bular capillaries, eNOS mRNA, and VEGF mRNA in the UUO group were significantly decreased compared with those of the sham group at 2, 3, and 4 weeks (P<0.05).NO was positively correlated with peritubular capillaries (r =0.715, P<0.05), eNOS mRNA was positively correlated with peritubular capillaries (r =0.624, P<0.05), endothelial progeni-tor cells (r =0.375, P<0.05), and VEGF mRNA (r =0.351, P<0.05).Conclusions eNOS/NO signaling path-way participates in regulation of peritubular capillary lesions in renal interstitial fibrosis of UUO mice .The mechanism may be partly related to the regulation of vasomotor reflex , the expression of VEGF mRNA and mobilization of endothelial pro-genitor cells.